Journal: Cell reports

Article Title: Disrupted population coding in the prefrontal cortex underlies pain aversion

doi: 10.1016/j.celrep.2021.109978

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Gradient-index lens , Inscopix , https://www.inscopix.com/lenses-viruses.

Techniques: Virus, Recombinant, Adjuvant, Software, Adhesive

Journal: The Journal of Neuroscience

Article Title: Shared Dorsal Periaqueductal Gray Activation Patterns during Exposure to Innate and Conditioned Threats

doi: 10.1523/JNEUROSCI.2450-20.2021

Figure Lengend Snippet: Characterization of recording quality in the dPAG. A, A viral vector was injected in the dPAG encoding GCaMP6s under the pan-neuronal syn promoter. A GRIN lens was implanted over the injection site and coupled to a miniaturized microscope to obtain recordings of calcium transients in the dPAG. B, Example traces of simultaneously recorded cells. C, Maximum projection of the dPAG FOV in a representative example mouse. D, Example imaging FOV with dPAG cells coregistered between Rat exposure 1 and 2. E, Bars represent the displacement of coregistered cells between Rat and Fear Acquisition assays as a fraction of the mean neuron diameter (n = 527). F, The peak-to-noise ratio (PNR) and mean peak amplitude correlation values were calculated for coregistered cells between Rat and Fear Acquisition assays. Cell identities were then shuffled within the 10 nearest neighbors 1000 times, and the same correlation measures were calculated for each iteration. The resulting bootstrap distribution was compared with the actual peak-to-noise and mean peak amplitude values, indicated with a blue arrow (n = 527; comparison of actual values to bootstrap distribution; p < 0.001).

Article Snippet: A 0.5-mm-diameter, ∼4-mm-long gradient refractive index (GRIN) lens (Inscopix) was implanted above the dPAG (−2.0 mm ventral to the skull surface).

Techniques: Plasmid Preparation, Injection, Microscopy, Imaging